| Annotated protein: | Calnexin. Gene symbol: CANX. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P35565 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CANX |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic membrane (GO:0099055) |
| Synapse type(s): | forebrain, glutamatergic |
| Annotated paper: | Itakura M, et al. "NMDA receptor-dependent recruitment of calnexin to the neuronal plasma membrane" Neurosci Lett. 2013 Aug 29;550:173-8 PMID:23851254 |
| Figure(s): | Fig. 2,3 |
| Annotation description: | Fig. 2,3: Calnexin colocalized with AMPAR clusters in the neuronal plasma membrane and can be biotinylated as cell surface protein. This indicates that in addition to its localization in the ER Calnexin can occur in synaptic membranes exposed to the cell surface. 29/3/2017 Pim: - AMPARs clusters on the neuronal surface are considered to represent synapses. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Western blot IP + WB/MSMS Protein-protein interaction (generic) |
| Annotator(s): | Daniela Dieterich (ORCID:0000-0002-9880-1214) Rainer Pielot (ORCID:0000-0002-9681-3318) Karl-Heinz Smalla (ORCID:0000-0002-0269-0311) Eckart Gundelfinger (ORCID:0000-0001-9377-7414) |
| Lab: | Leibniz Institute for Neurobiology (LIN), Institute of Pharmacology and Toxicology, Otto von Guericke University, Center for Behavioral Brain Sciences (CBBS), Magdeburg, Germany |
| Additional literature: | Calnexin is a type I endoplasmic reticulum integral membrane protein and responsible for the folding and quality control of newly-synthesized (glyco)proteins. To date its function has been assigned exclusively to ER-related compartments. @ PMID:26676362 Immunoblot analysis of SDS-PAGE gels of total detergent-solubilized membrane fractions immunoprecipitated (IP) with antibodies to the AMPA receptor subunits GluR1-4 and to calnexin (C-terminus antibody) and BiP. As Calnexin is considered as a ER component this interaction is supposed to occur at the postsynaptic membrane. @ PMID:10461883 |
| SynGO annotation ID: | 295 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |