| Annotated protein: | Rho guanine nucleotide exchange factor 15 (Ephexin-5) (E5). Gene symbol: ARHGEF15. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q5FWH6 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ARHGEF15 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynapse assembly (GO:0150052) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Hamilton AM, et al. "A dual role for the RhoGEF Ephexin5 in regulation of dendritic spine outgrowth" Mol Cell Neurosci. 2017 Apr;80:66-74 PMID:28185854 |
| Figure(s): | Fig.1, 4 |
| Annotation description: | Fig.1: "Ephexin5 inhibits spine outgrowth in a GEF-dependent manner" - "We found that shRNA-mediated knockdown of E5 led to increased spine outgrowth (0.62 ± 0.02 spines/10 μm/15 min) relative to EGFP alone (0.43 ± 0.04 spines/10 μm/15 min; p < 0.001) or scrambled shRNA (0.42 ± 0.06 spines/10 μm/15 min; p < 0.05; Fig. 1B)." Fig.4: "Ephexin5 is necessary for activity-induced spine outgrowth" - "To test whether E5 is necessary for activity-dependent spine outgrowth, we treated hippocampal slices from WT and E5 KO (Margolis et al., 2010) mice with vehicle or 30 μM bicuculline. As expected, in WT neurons, spine outgrowth increased following bicuculline treatment (0.53 ± 0.07 spines/10 μm/15 min) relative to vehicle-treated controls (0.28 ± 0.02 spines/10 μm/15 min; p < 0.05; Fig. 4A, B). In contrast, there was no increase in spine outgrowth on E5 KO cells treated with bicuculline (0.31 ± 0.02 spines/10 μm/15 min) over that observed on their vehicle-treated counterparts (0.31 ± 0.06 spines/10 μm/15 min; p = 0.9; Fig. 4A, B). Thus, surprisingly, Ephexin5 both restricts spine outgrowth and is necessary for activity-dependent increases in spine outgrowth." |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) RNAi / shRNA |
| Evidence tracking, Experiment Assay: | Two-photon microscopy |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2942 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |