| Annotated protein: | Small ribosomal subunit protein eS6 (40S ribosomal protein S6). Gene symbol: RPS6. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P62755 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ RPS6 |
| Ontology domain: | Cellular Component |
| SynGO term: | presynaptic ribosome |
| Synapse type(s): | hippocampus, GABAergic |
| Annotated paper: | Younts TJ, et al. "Presynaptic Protein Synthesis Is Required for Long-Term Plasticity of GABA Release" Neuron. 2016 Oct 19;92(2):479-492 PMID:27764673 |
| Figure(s): | Fig.7 |
| Annotation description: | Fig.7: "Ribosomes are present in CB1-expressing interneuron axons and terminals" - "In a complementary set of experiments, we immunolabeled ribosomal protein S6 (rpS6), which is an integral component of the eukaryotic 40S ribosomal subunit and an effector of mTOR signaling. rpS6 molecules were also readily visualized with STORM imaging inside CB1-expressing axon terminals (Figure 7C, Figure S5C). The densities of 5.8S rRNA (Figure 7D) and rpS6 protein (Figure 7E) puncta were significantly higher (by ~90%) in axon terminals compared to the corresponding background signal measured from CA1 pyramidal cell nuclei. " - "Taken together, these findings provide molecular and anatomical evidence for the presence of presynaptic ribosomes inside CB1-expressing interneuron terminals." |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Super resolution |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2940 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |