| Annotated protein: | LHFPL tetraspan subfamily member 4 protein (GABAA receptor regulatory Lhfpl4) (Lipoma HMGIC fusion partner-like 4 protein). Gene symbol: LHFPL4. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q5U4E0 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ LHFPL4 |
| Ontology domain: | Biological Process |
| SynGO term: | neurotransmitter receptor localization to postsynaptic specialization membrane (GO:0099645) |
| Synapse type(s): | hippocampus, GABAergic |
| Annotated paper: | Yamasaki T, et al. "GARLH Family Proteins Stabilize GABA(A) Receptors at Synapses" Neuron. 2017 Mar 8;93(5):1138-1152.e6 PMID:28279354 |
| Figure(s): | Fig.5, 7, 8 |
| Annotation description: | Background: Lhfpl4 is a transmembrane protein that is shown to 1) interact with GABAaRs and mediate the synaptic localization of these receptors. The authors hypothesize that Lhfpl4 should be cosidered an accesory protein like TARPs Fig.5: "Lhfpl4 is essential for synaptic GABAAR clustering" - "The γ2 subunit was co-localized with inhibitory presynaptic GAD65 in neurons treated with lentivirus containing only GFP (Mock, Figure 5B). The number of γ2-immunoreactive puncta was markedly reduced in LH4 shRNA-treated neurons (Figure 5B and C and Figure S3A) without changes in the size or signal intensity of the remaining puncta (Figure 5C)." - Since there are still GABARs present, but many of the synaptic puncta are removed this should point to less clustering of GABAaRs at postsynapse, not less GABAaRs on the plasma membrane. Fig.7: "Lhfpl4 is required for GABAAR-mediated synaptic transmission" Fig.8: "GABAAR-mediated synaptic transmission requires LH4 in adult hippocampus" |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA |
| Evidence tracking, Experiment Assay: | Confocal Whole-cell patch clamp |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2937 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |