| Annotated protein: | CLPTM1 regulator of GABA type A receptor forward trafficking (Cleft lip and palate associated transmembrane protein 1). Gene symbol: CLPTM1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: A6J8Q9 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CLPTM1 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynaptic membrane neurotransmitter receptor levels (GO:0099072) |
| Synapse type(s): | hippocampus, GABAergic |
| Annotated paper: | Ge Y, et al. "Clptm1 Limits Forward Trafficking of GABA(A) Receptors to Scale Inhibitory Synaptic Strength" Neuron. 2018 Feb 7;97(3):596-610.e8 PMID:29395912 |
| Figure(s): | Fig.4, 5, 6 |
| Annotation description: | Fig.4: "Overexpression of Clptm1 Scales Down Inhibitory Synaptic Strength" - "To directly assess whether overexpression of Clptm1 reduces synaptic GABAARs, we immunostained for surface GABAAR γ2 and for vesicular GABA transporter (VGAT), an inhibitory presynaptic marker. Consistent with the reduction in amplitude, but not frequency, of mIPSCs (Figure 4C), overexpression of Clptm1 reduced the intensity but not the number of postsynaptic surface clusters of GABAAR γ2 (Figure 4D). Overexpression of Clptm1 did not alter the intensity or number of postsynaptic clusters of gephyrin or neuroligin-2 (Figures 4E and 4F), indicating a specific effect on GABAARs." Fig.5: "Knockdown of Clptm1 Scales Up Inhibitory Synaptic Strength" - " The increase in mIPSC amplitude by Clptm1 knockdown was restored back to control level by co-expression of an RNAi-resistant Clptm1* (Figure 5A)," - " Consistent with the effect on mIPSC amplitude, knockdown of Clptm1 resulted in an increase in the intensity of surface synaptic GABAAR γ2 clusters (Figure 5C). " Fig.6: "Clptm1 Modulates Inhibitory Synaptic Transmission In Vivo" Note: Clptm1 is identified as a GABAR accessory protein that regulates the surface expression of these receptors. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA Over-expression |
| Evidence tracking, Experiment Assay: | Confocal Whole-cell patch clamp |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2932 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |