| Annotated protein: | Protein numb homolog (m-Nb) (m-Numb). Gene symbol: NUMB. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q9QZS3 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ NUMB |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynaptic neurotransmitter receptor endocytosis (GO:0099149) |
| Synapse type(s): | cerebellum, glutamatergic |
| Annotated paper: | Zhou L, et al. "Numb deficiency in cerebellar Purkinje cells impairs synaptic expression of metabotropic glutamate receptor and motor coordination" Proc Natl Acad Sci U S A. 2015 Dec 15;112(50):15474-9 PMID:26621723 |
| Figure(s): | Fig.2, 3, 5 |
| Annotation description: | Fig.2: Inhibited LTD but normal LTP in Numb-cKO mice" Fig.3: "Synaptic mGlu1 is reduced in Numb-cKO mice" Fig.5: "mGlu1-LTD and mGlu1-STP are normal, but mGlu1 trafficking is inhibited in Numb-cKO mice" - "It has been shown that mGlu1 is internalized (63-66) and recycled back into the cell membrane (65) upon ligand exposure. Because trafficking may affect receptor targeting at the cell membrane, we investigated whether Numb is involved in the internalization and recycling of mGlu1. DHPG (100 μM) was applied to acute cerebellar slices for 10 min, and the level of synaptic mGlu1 was measured 30 min (for endocytosis) or 180 min (for recycling) after DHPG application. In control slices, synaptic mGlu1 decreased to 34 ± 4% of the basal level at t = 40 min (n = 4; P < 0.01) but returned to 77 ± 8% at t = 190 min (n = 4; P > 0.05) (Fig. 5 E and F). The down- and up-regulation of mGlu1 expression in response to such agonist challenges was similar to results reported in previous work in other wild-type mice (66-68). In contrast, synaptic mGlu1 was not changed either 30 min or 180 min after DHPG application to Numb-cKO slices (t = 40 min: 96 ± 8%; t = 190 min: 103 ± 8%; P > 0.05 compared with baseline) (Fig. 5 E and F), suggesting that Numb is required for the internalization and recycling of mGlu1 in PCs." |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) Antibody (detection) |
| Evidence tracking, Experiment Assay: | Whole-cell patch clamp Western blot Biochemical fractionation (generic) |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2927 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |