Annotated protein:Serine/threonine kinase 38 like. Gene symbol: STK38L. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: A4GW50
antibody wiki:
SynGO gene info:SynGO data @ STK38L
Ontology domain:Biological Process
SynGO term:postsynapse organization (GO:0099173)
Synapse type(s):hippocampus, glutamatergic
Annotated paper:Ultanir SK, et al. "Chemical genetic identification of NDR1/2 kinase substrates AAK1 and Rabin8 Uncovers their roles in dendrite arborization and spine development" Neuron. 2012 Mar 22;73(6):1127-42 PMID:22445341
Figure(s):Fig.3, 4
Annotation description:Fig.3: "NDR1/2's role on dendritic spine and excitatory postsynaptic development"
- "Expression of dominant negative NDR1 (NDR1-KD or NDR1-AA) caused a robust increase of filopodia and atypical protrusion density together with a reduction in mushroom spine density (Figure 3A-C), indicating that NDR1 function is necessary for mushroom spine formation. In contrast, NDR1-CA drastically reduced the total dendritic protrusion density, as a result of significant reduction in mushroom spines, filopodia and stubby spines (Figure 3A-C). "
- "Similar to the dominant negative effects of NDR1 mutants, NDR1siRNA + NDR2siRNA also resulted in increased filopodia and atypical protrusions and decreased mushroom spine densities, which was rescued by co-expression of siRNA resistant NDR1 (NDR1*) (Figure 3A & D)"

Fig.4: "In vivo analysis of NDR1 mutants and siRNA by in utero electroporation"
- " We found that NDR1-KD and NDR1siRNA + NDR2siRNA decreased dendritic spine head diameter (Figure 4G), suggesting that NDR1/2 is required for dendritic spine development in vivo"
Evidence tracking, Biological System:Intact tissue
Cultured neurons
Evidence tracking, Protein Targeting:RNAi / shRNA
Over-expression
Evidence tracking, Experiment Assay:Confocal
Annotator(s):Pim van Nierop (ORCID:0000-0003-0593-3443)
Guus Smit (ORCID:0000-0002-2286-1587)
Matthijs Verhage (ORCID:0000-0002-2514-0216)
Lab:Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands
SynGO annotation ID:2926
Dataset release (version):20231201
View annotation as GO-CAM model:Gene Ontology