Annotated protein:Oligophrenin-1. Gene symbol: OPHN1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P0CAX5
antibody wiki:
SynGO gene info:SynGO data @ OPHN1
Ontology domain:Biological Process
SynGO term:maintenance of postsynaptic specialization structure (GO:0098880)
Synapse type(s):hippocampus, glutamatergic
Annotated paper:Nakano-Kobayashi A, et al. "The X-linked mental retardation protein OPHN1 interacts with Homer1b/c to control spine endocytic zone positioning and expression of synaptic potentiation" J Neurosci. 2014 Jun 25;34(26):8665-71 PMID:24966368
Figure(s):Fig.1, 2, 3, 4
Annotation description:Fig.1: "OPHN1-Homer interaction is critical for LTP"
- "Whereas LTP-inducing stimuli produced a robust and long-lasting potentiation in control neurons, only an initial, transient increase in synaptic transmission was observed in simultaneously recorded neurons coexpressing OPHN1#2 shRNA and OPHN1Hom (Fig. 1A)."

Fig.2: "OPHN1-Homer interaction is required for positioning EZ near PSD"
- "Consistent with previous findings (Lu et al., 2007), we observed that in scr#1 shRNA-expressing neurons PSD-95-GFP puncta were nearly always associated with clathrin-DsRed (Fig. 2A). In OPHN1#2 shRNA-expressing neurons, however, we noted a considerable loss of PSD-associated clathrin puncta (Fig. 2A)."

Fig.3: "Disruption of OPHN1-Homer interaction reduces the number of newly exocytosed AMPARs at synaptic sites in basal synaptic conditions"
- "GluA1 exocytosis at synaptic sites was significantly reduced in neurons treated with pep-OPHN1Hom, compared with pep-contHom or no peptide-treated neurons (Fig. 3C,D). "

Fig.4: "OPHN1-Homer interaction is essential for glycine-induced increase in receptor recycling/exocytosis and synaptic AMPAR number"
- "glycine induced a robust increase in the amount of SEP-GluA1 at the spine surface of these neurons (Fig. 4A-C). In contrast, when neurons were pretreated with pep-OPHN1Hom, only a modest increase in synaptic SEP-GluA1 was observed upon glycine stimulation (Fig. 4A-C)..."
Evidence tracking, Biological System:Intact tissue
Cultured neurons
Evidence tracking, Protein Targeting:RNAi / shRNA
Over-expression
Antagonist / agonist
Evidence tracking, Experiment Assay:Confocal
Whole-cell patch clamp
Optical physiology
Annotator(s):Pim van Nierop (ORCID:0000-0003-0593-3443)
Guus Smit (ORCID:0000-0002-2286-1587)
Matthijs Verhage (ORCID:0000-0002-2514-0216)
Lab:Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands
SynGO annotation ID:2919
Dataset release (version):20231201
View annotation as GO-CAM model:Gene Ontology