| Annotated protein: | Protein kinase C and casein kinase substrate in neurons protein 1 (Dynamin proline-rich domain-interacting protein) (Dynamin PRD-interacting protein) (Synaptic, dynamin-associated protein I) (Syndapin-1) (Syndapin-I) (SdpI). Gene symbol: PACSIN1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q9Z0W5 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PACSIN1 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynaptic neurotransmitter receptor endocytosis (GO:0099149) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Anggono V, et al. "PICK1 interacts with PACSIN to regulate AMPA receptor internalization and cerebellar long-term depression" Proc Natl Acad Sci U S A. 2013 Aug 20;110(34):13976-81 PMID:23918399 |
| Figure(s): | Fig.3, 4 |
| Annotation description: | Fig.3: "PACSIN1 knockdown reduces NMDA-induced GluA2 internalization" - "These data indicate that PACSIN1 may not regulate AMPAR trafficking under basal conditions." - " the amount of internalized myc-GluA2 was significantly reduced in PACSIN1 knockdown neurons compared with control neurons (internalization index, pSuper, 1 ± 0.04; sh#1, 0.68 ± 0.03) (Fig. 3 E and F). The reduction in NMDA-induced myc-GluA2 internalization caused by PACSIN1 shRNA could be fully rescued by the expression of an shRNA-resistant PACSIN1 rescue construct in the hippocampal neurons (0.92 ± 0.05) (Fig. 3 E and F). " Fig.4: "PICK1 binding to the PACSIN1-variable region is required for activity-dependent GluA2 internalization" - "there was a significant decrease in intracellular myc-GluA2 following NMDA stimulation in neurons that were transfected with HA-PACSIN1 tmE, which had reduced binding with PICK1 (0.84 ± 0.03) (Fig. 4 D and E)." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA Over-expression |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2907 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |