| Annotated protein: | Phosphatidylinositol 4-phosphate 5-kinase type-1 gamma (PIP5K1-gamma) (PtdIns(4)P-5-kinase 1 gamma) (EC 2.7.1.68) (Phosphatidylinositol 4-phosphate 5-kinase type I gamma) (PIP5KIgamma). Gene symbol: PIP5K1C. Taxonomy: Mus musculus (Mouse). Uniprot ID: O70161 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PIP5K1C |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density (GO:0014069) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Unoki T, et al. "NMDA receptor-mediated PIP5K activation to produce PI(4,5)P(2) is essential for AMPA receptor endocytosis during LTD" Neuron. 2012 Jan 12;73(1):135-48 PMID:22243752 |
| Figure(s): | Fig.1 |
| Annotation description: | Fig.1: "Expression of PIP5Kγ661 in Hippocampal Neurons" - "Like postsynaptic density 95 (PSD-95) and filamentous actin (F-actin), which were concentrated in the dendritic spines, endogenous PIP5Kγ661 was enriched in dendritic spine-like protrusions (see Figures S1A-S1E available online). Endogenous PIP5Kγ661 partially colocalized with PSD-95 and F-actin (Figures 1D and 1E). Furthermore, immunoblot analysis of the subcellular fractions of adult mouse brain showed PIP5Kγ661 not only in the SV fraction, which was immunonegative for PSD-95, but also in the PSD fractions, which were immunonegative for an SV marker synaptophysin (Figure 1F). Together, these results indicate that PIP5Kγ661 localizes at least in part to postsynapses." |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Western blot Biochemical fractionation (generic) |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2904 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |