| Annotated protein: | Activity-regulated cytoskeleton-associated protein (mArc) (Activity-regulated gene 3.1 protein) (ARC/ARG3.1) (Arg3.1). Gene symbol: ARC. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q9WV31 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ARC |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynaptic neurotransmitter receptor endocytosis (GO:0099149) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Chowdhury S, et al. "Arc/Arg3.1 interacts with the endocytic machinery to regulate AMPA receptor trafficking" Neuron. 2006 Nov 9;52(3):445-59 PMID:17088211 |
| Figure(s): | Fig.6, 7, 8, 9 |
| Annotation description: | Fig.6: "Arc Expression Decreases Surface and Total AMPARs" - "Arc transgene expression resulted in a marked reduction of surface GluR1 (Figure 6A and B). This action was dependent on Arc's ability to bind endophilin since Arc (Δ91-100) had no effect on surface GluR1 (Figure 6A and B)" Fig.7: "The Arc-Dynamin Interaction is Required for the Arc-dependent Decrease in Surface AMPARs" - "A deletion mutant of Arc (Δ195-214) that does not bind dynamin also had no effect on surface GluR1 (Figure 7B), suggesting that both the endophilin and dynamin binding domains are necessary for Arc's modulation of AMPAR trafficking." Fig.8: "Arc Increases AMPAR Endocytosis while Arc KO Neurons Exhibit Increased Surface AMPARs and a Deficit in Endocytosis" - "Despite markedly reduced surface GluR1, the amount of internalized GluR1 was similar to that seen in control neurons (Figure 8A, C and S9). Accordingly, the percent of surface GluR1 internalized was increased in neurons that express Arc. We conclude that Arc increases the rate of GluR1 endocytosis." Fig.9: "Arc Expression in Arc KO Neurons Rescues Surface GluR1 Levels" - "Arc expressing neurons significantly downregulated surface GluR1 levels compared to surrounding untransfected neurons (Figure 9)." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) Over-expression |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2903 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |