| Annotated protein: | Activity-regulated cytoskeleton-associated protein (Activity-regulated gene 3.1 protein) (ARC/ARG3.1) (Arg3.1). Gene symbol: ARC. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q63053 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ARC |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density membrane (GO:0098839) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Chowdhury S, et al. "Arc/Arg3.1 interacts with the endocytic machinery to regulate AMPA receptor trafficking" Neuron. 2006 Nov 9;52(3):445-59 PMID:17088211 |
| Figure(s): | Fig.2, S4A |
| Annotation description: | Fig.2: "Endophilin 2, 3 and Arc Localize to Post-synaptic Sites with GluR1 in Hippocampal Neurons" - "Arc immuno-EM gold particles were present throughout the spine but were enriched over the postsynaptic density (PSD) where they appeared at, or very near, the plasma membrane (Figure 2B)." - "Consistent with EM localizations, Arc protein localized in DIV 21 hippocampal primary neurons to dendrites with puncta in spines, and colocalized with both the AMPAR subunit GluR1 and endophilin 3 (Figures 2C)." Supplemental Figure S4A: "Arc and Endophilin Associate with Endosomes in Neurons" - "When Arc was expressed individually, the transgene accumulates in dendrites with a uniform, dispersed distribution, and shows enrichment in dendritic spines (Supplemental Figure S4A)" |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Confocal |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2900 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |