| Annotated protein: | Myosin-10 (Cellular myosin heavy chain, type B) (Myosin heavy chain 10) (Myosin heavy chain, non-muscle IIb) (Non-muscle myosin heavy chain B) (NMMHC-B) (Non-muscle myosin heavy chain IIb) (NMMHC II-b) (NMMHC-IIB). Gene symbol: MYH10. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q61879 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ MYH10 |
| Ontology domain: | Biological Process |
| SynGO term: | postsynaptic actin cytoskeleton organization (GO:0098974) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Yagi H, et al. "Filamin A-interacting protein (FILIP) is a region-specific modulator of myosin 2b and controls spine morphology and NMDA receptor accumulation" Sci Rep. 2014 Sep 15;4:6353 PMID:25220605 |
| Figure(s): | Supplemental Fig. S3, S6 |
| Annotation description: | Supplemental Fig. S3: "FILIP regulates the subcellular distribution of NMHC IIb and modulates NMHC IIb binding to F-actins" Supplemental Fig. S6: "Knockdown of NMHC IIb results in the elongation of spine length" - "Whereas the stress fibre-like distribution of myosin 2b was dominant in COS-7 cells without endogenous FILIP, the number of cells with granular myosin 2b distribution surpassed that of cells with stress fibre-like distribution in the presence of FILIP (see Supplemental Fig. S3a, b online). We next investigated whether FILIP influenced the formation of actin stress fibres, as myosin 2 is a component of actin stress fibres21,22. The number of cells with actin stress fibres decreased in the presence of FILIP (see Supplemental Fig. S3c, d online). Because FILIP binds to the N-terminal domain of myosin 2b, which is essential for binding to actin fibres, and the binding of myosin 2b to actin fibres is essential for the function of myosin 2b, we hypothesised that FILIP interferes with the activity of myosin 2b. We then compared the appearance of NMHC IIb in COS-7 cells in the presence of the myosin 2b inhibitor blebbistatin. Blebbistatin binds to the myosin globular head domain and blocks the rigid binding of myosin 2b to actin23. " |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2892 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |