| Annotated protein: | Myosin-10 (Cellular myosin heavy chain, type B) (Myosin heavy chain 10) (Myosin heavy chain, non-muscle IIb) (Non-muscle myosin heavy chain B) (NMMHC-B) (Non-muscle myosin heavy chain IIb) (NMMHC II-b) (NMMHC-IIB). Gene symbol: MYH10. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q9JLT0 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ MYH10 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic actin cytoskeleton (GO:0098871) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Korobova F, et al. "Molecular architecture of synaptic actin cytoskeleton in hippocampal neurons reveals a mechanism of dendritic spine morphogenesis" Mol Biol Cell. 2010 Jan 1;21(1):165-76 PMID:19889835 |
| Figure(s): | Fig.4, 5 |
| Annotation description: | Fig.4: "Immunofluorescence staining of cytoskeletal proteins in spines" - Immunofluorescence staining revealed a punctate, or sometimes diffuse, distribution of myosin II in dendritic spines, dendrites, and the cell body (Figure 4, C and F). ...In spines, myosin II puncta predominantly localized to the neck and a lower part of the head, and to a lesser extent to the base. Fig.5: "Immunogold EM of cytoskeleton-associated proteins in spines" - "Immunogold EM revealed similar distribution of myosin II between spine compartments (Figure 5D). Thus, of 11 mushroom or thin spines analyzed, the neck (10 spines) and the lower part of the head (9 spines) were most frequently labeled, whereas the base labeling was less frequent (5 spines). However, gold particles in the neck frequently formed linear clusters, possibly reflecting formation of myosin II filaments, whereas in the head they were usually scattered as single particles (Figure 5D)." |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Confocal |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2891 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |