| Annotated protein: | DnaJ (Hsp40) homolog, subfamily B, member 1 (Predicted), isoform CRA_b (Dnajb1 protein). Gene symbol: DNAJB1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: B0K030 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ DNAJB1 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Synapse type(s): | cerebral cortex, glutamatergic |
| Annotated paper: | Suzuki T, et al. "Presence of molecular chaperones, heat shock cognate (Hsc) 70 and heat shock proteins (Hsp) 40, in the postsynaptic structures of rat brain" Brain Res. 1999 Jan 16;816(1):99-110 PMID:9878698 |
| Figure(s): | Fig.2, 7, 10 |
| Annotation description: | Fig.2: "Detection of HSP70 and Hsp40 in the PSD fractions prepared from various parts of the rat brain" - "The Hsp40 was highly concentrated in the PSD fraction, less abundant in the crude nuclear pellet (P1), and was barely detected in the synaptosome and synaptic plasma membrane (SM) fractions. The distribution of synaptophysin, a presynaptic marker in the synaptic vesicle membrane, was also compared with that of Hsp40. The distribution of the two proteins was different." Fig.8: "localization of Hsp40 immunoreactivity in the synaptic areas at an electron microscopic level" - "Postsynaptic structures but not presynaptic structures were stained (Fig. 8). PSDs were immunoreactive but rather weak compared to regions of subsynaptic web. Staining patterns of HSP70 and Hsp40 in the spine were similar" Fig.10: " Comparison of the distribution of Hsp40 and synaptophysin by double immunostaining and confocal microscopic examination" - "Synaptophysin immunoreactivity was distributed in a patch-like pattern in cultured neurons, to which Hsp40 was localized. Patchy stainings of synaptophysin may represent the localization of presynaptic terminals." - Note: this confirms synaptic localization of Dnajb1; Hsp40. Note: - The fractionation and EM data are not in agreement. Fractionation shows an exclusive localization of Dnajb1; Hsp40 in the PSD fraction whereas EM shows most abundant signals is other postsynaptic regions. I have annotated to the more conservative term 'postsynapse (GO:0098794)'. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Western blot Biochemical fractionation (generic) |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2888 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |