| Annotated protein: | Heat shock cognate 71 kDa protein (EC 3.6.4.10) (Heat shock 70 kDa protein 8). Gene symbol: HSPA8. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P63018 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ HSPA8 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density (GO:0014069) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Moon IS, et al. "Presence of both constitutive and inducible forms of heat shock protein 70 in the cerebral cortex and hippocampal synapses" Cereb Cortex. 2001 Mar;11(3):238-48 PMID:11230095 |
| Figure(s): | Fig.2, 5, 8 |
| Annotation description: | Fig.2: "Association properties of HSP70 with the PSD" - "As shown in Figure 2C, Hsp70 and the lower band of Hsc70 are not specifically enriched in the PSD fraction. Interestingly, the amount of the upper band (75 kDa) of Hsc70 is dramatically reduced (14.1 ± 2.2%, n = 2, that of synaptosome) in the One-Triton PSD fraction (Fig. 2C, Hsc70, upper arrowhead), indicating that the majority of this protein is removed by 0.5% Triton X-100 and that it may not be an intrinsic member of the PSD." Fig.5: "Expression of Hsp70 in dissociated hippocampal neurons" - "The subcellular distribution of Hsc70 immunoreactivity was similar to that of Hsp70 in that most of the puncta were double-labeled with both Hsc70 and PSD-95 antibodies (Fig. 6A)" Fig.8: "Immunoelectron microscopic localization of Hsp70 in synaptic sites" - "Immunoreactive structures for Hsc70 were similar to those staining for Hsp70. Asymmetrical synapses (Fig. 8, asterisks), including axospinous ones (Fig. 8B, asterisk), were strongly stained, but symmetrical ones were stained weakly (Fig. 8A, large open arrows). Presynaptic thickenings and synaptic vesicles were stained weakly. Patchy staining was scattered throughout the cytoplasm." |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Confocal Western blot Biochemical fractionation (generic) |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2884 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |