| Annotated protein: | Epidermal growth factor receptor pathway substrate 15. Gene symbol: EPS15. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: E9PSY8 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ EPS15 |
| Ontology domain: | Biological Process |
| SynGO term: | postsynaptic neurotransmitter receptor endocytosis (GO:0098884) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Lin A, et al. "Endocytic adaptor epidermal growth factor receptor substrate 15 (Eps15) is involved in the trafficking of ubiquitinated alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors" J Biol Chem. 2014 Aug 29;289(35):24652-64 PMID:25023288 |
| Figure(s): | Fig.3-9 |
| Annotation description: | Fig.3: "Eps15 regulates GluA1 surface expression in neurons" Fig.4: "Eps15 regulates basal AMPAR endocytic trafficking" Fig.5: "Eps15 regulates glutamate-induced AMPAR endocytic trafficking" Fig.6: "Eps15 and GluA1 interaction is ubiquitin-dependent" Fig.7: "GluA1 ubiquitination is required for Eps15 association" Fig.8: "Nedd4 enhances Eps15 association with GluA1" Fig.9: "Eps15-mediated AMPAR internalization is clathrin-dependent" Literal: "AMPAR internalization begins with an association with the adaptor protein AP2, or Eps15 for the ubiquitinated receptors, followed by the recruitment of clathrin to form clathrin-coated pits. Clathrin interacts with amphiphysin, which then brings in dynamin to subsequently pinch off the coated pits to form endocytic vesicles." |
| Evidence tracking, Biological System: | Cultured neurons Non-neuronal tissue |
| Evidence tracking, Protein Targeting: | RNAi / shRNA Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Western blot IP + WB/MSMS |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2883 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |