| Annotated protein: | Epidermal growth factor receptor pathway substrate 15. Gene symbol: EPS15. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: E9PSY8 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ EPS15 |
| Ontology domain: | Cellular Component |
| SynGO term: | synapse (GO:0045202) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Lin A, et al. "Endocytic adaptor epidermal growth factor receptor substrate 15 (Eps15) is involved in the trafficking of ubiquitinated alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors" J Biol Chem. 2014 Aug 29;289(35):24652-64 PMID:25023288 |
| Figure(s): | Fig.1 |
| Annotation description: | Fig.1: "Synaptic localization of Eps15" - "Eps15 immunosignals were detected throughout the neuron but formed intense clusters along the dendrites, partially colocalizing with the postsynaptic density scaffolding molecule PSD-95 (Fig. 1A). Similarly, most of the Eps15 clusters appeared to codistribute with GluA1 along the dendrites (Fig. 1B), indicating a synaptic localization of Eps15. To further examine the relative protein distribution of Eps15 at synapses, we prepared synaptosomes from both adult rat brain lysate and cultured rat cortical cell lysates. Western blotting demonstrated a strong enrichment of Eps15 in synaptosomal preparations compared with cell lysates (Fig. 1, C and D)" |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2882 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |