| Annotated protein: | Dynamin-2 (EC 3.6.5.5). Gene symbol: DNM2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P39052 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ DNM2 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density, intracellular component (GO:0099092) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Okamoto PM, et al. "Dynamin isoform-specific interaction with the shank/ProSAP scaffolding proteins of the postsynaptic density and actin cytoskeleton" J Biol Chem. 2001 Dec 21;276(51):48458-65 PMID:11583995 |
| Figure(s): | Fig.1, 2, 3, 4, 5, 6 |
| Annotation description: | Fig.1: "Dynamin isoform-specific association with Shank/ProSAP proteins and mapping of the binding sites via the yeast two-hybrid assay" Fig.2: "Co-immunoprecipitation of dynamin and Shank in COS7 cells" - "...t dynamin-2 co-immunoprecipitates Shank2 more efficiently than does dynamin-1. Both dynamin isoforms co-immunoprecipitated Myc-tagged Shank1 (Fig. 2b), and, again, overexpressed dynamin-2 performed better than dynamin-1." Fig.3: "Dynamin-2 interacts directly with the Shank/ProSAP family members" - "To ascertain whether the interaction between dynamin-2 and the Shank family is, indeed, direct, a GST fusion protein containing the region of Shank2 corresponding to the initially identified prey fragment was tested for its ability to bind purified dynamin-2. Clear binding by the GST-Shank2 fusion protein, but not by GST alone, was observed (Fig. 3a)." Fig.4: "Immunofluorescence microscopy of dynamin isoforms in hippocampal neurons" - "Immunofluorescence microscopy of cultured hippocampal neurons revealed punctate staining along dendrites with both the dynamin-2 and dynamin-1b carboxyl-terminal isoform-specific antibodies (Fig. 4). The dynamin-2 staining exhibited clear co-localization with the synaptic marker synaptophysin (Fig. 4)." Fig.5: "Specific association of dynamin-2 with postsynaptic densities" - "Both dynamin-1 and -2 isoforms were found in the synaptosomal fraction. However, dynamin-2 was clearly found in the purified PSD fraction, whereas dynamin-1 was present at low to undetectable amounts (Fig. 5)." Fig.6: "Co-expression of PSD proteins" - "However, triple expression of dynamin-2 with both GKAP1a/SAPAP and Shank1a/ProSAP2 resulted in significant co-localization of the three proteins into clusters that were reminiscent of those formed by overexpressing PSD95 and Shaker K+ channels in COS cells (Fig. 6 and Ref. 53)." |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons Non-neuronal tissue |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Wide-field fluorescence Western blot Biochemical fractionation (generic) IP + WB/MSMS Y2H |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2880 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |