| Annotated protein: | Neuron-specific vesicular protein calcyon. Gene symbol: CALY. Taxonomy: Homo sapiens (Human). Uniprot ID: Q9NYX4 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CALY |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic endocytic zone (GO:0098843) |
| Synapse type(s): | neocortex |
| Annotated paper: | Xiao J, et al. "Calcyon, a novel partner of clathrin light chain, stimulates clathrin-mediated endocytosis" J Biol Chem. 2006 Jun 2;281(22):15182-93 PMID:16595675 |
| Figure(s): | Fig.2, 3 |
| Annotation description: | Fig.2: "Ultrastructural localization of calcyon in the macaque prefrontal cortex" - "vesicular intracellular organelles indicative of the endocytic and/or recycling machinery (28, 29) were labeled in extrasynaptic and intracellular regions of spines (Fig. 2B), dendrites (Fig. 2D), and axon terminals (Fig. 2C).The association of calcyon immunoreactivity to the intracellular side of the plasma membrane (Fig. 2, B-D) and the absence of labeling of the multivesicular bodies (MVB; Fig. 2, B and D) and of the synaptic membrane specialization of axon terminals and dendrites (Fig. 2, B-D) further support the observation that calcyon specifically localizes to intracellular endocytic pathways within neurons of the primate prefrontal cortex. Collectively, the above EM data highlight the potential for calcyon to regulate clathrin-mediated vesicle trafficking in neurons at several points along the endocytic and late secretory pathways (30)." Fig.3: "Association of calcyon with LC, and CCVs in mammalian cells" - "Further, there was a high degree of overlap in the LC and calcyon puncta in neuronal dendrites, spines, and cell bodies (Fig. 3, A-C). Quantitation of the dendritic distribution revealed that the vast majority (~80%) of calcyon-labeled puncta are also labeled by LC or abut LC-labeled puncta (Fig. 3D)." |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons Non-neuronal tissue |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Confocal IP + WB/MSMS |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | See Fig.2+3 for additional EM data @ PMID:18539394 |
| SynGO annotation ID: | 2875 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |