| Annotated protein: | Oligophrenin-1. Gene symbol: OPHN1. Taxonomy: Homo sapiens (Human). Uniprot ID: O60890 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ OPHN1 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynaptic neurotransmitter receptor endocytosis (GO:0099149) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Nakano-Kobayashi A, et al. "The X-linked mental retardation protein OPHN1 interacts with Homer1b/c to control spine endocytic zone positioning and expression of synaptic potentiation" J Neurosci. 2014 Jun 25;34(26):8665-71 PMID:24966368 |
| Figure(s): | Fig.1, 2, 3, 4 |
| Annotation description: | Fig.1: "OPHN1-Homer interaction is critical for LTP" - "Whereas LTP-inducing stimuli produced a robust and long-lasting potentiation in control neurons, only an initial, transient increase in synaptic transmission was observed in simultaneously recorded neurons coexpressing OPHN1#2 shRNA and OPHN1Hom (Fig. 1A)." Fig.2: "OPHN1-Homer interaction is required for positioning EZ near PSD" - "Consistent with previous findings (Lu et al., 2007), we observed that in scr#1 shRNA-expressing neurons PSD-95-GFP puncta were nearly always associated with clathrin-DsRed (Fig. 2A). In OPHN1#2 shRNA-expressing neurons, however, we noted a considerable loss of PSD-associated clathrin puncta (Fig. 2A)." Fig.3: "Disruption of OPHN1-Homer interaction reduces the number of newly exocytosed AMPARs at synaptic sites in basal synaptic conditions" - "GluA1 exocytosis at synaptic sites was significantly reduced in neurons treated with pep-OPHN1Hom, compared with pep-contHom or no peptide-treated neurons (Fig. 3C,D). " Fig.4: "OPHN1-Homer interaction is essential for glycine-induced increase in receptor recycling/exocytosis and synaptic AMPAR number" - "glycine induced a robust increase in the amount of SEP-GluA1 at the spine surface of these neurons (Fig. 4A-C). In contrast, when neurons were pretreated with pep-OPHN1Hom, only a modest increase in synaptic SEP-GluA1 was observed upon glycine stimulation (Fig. 4A-C)..." |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA Over-expression Antagonist / agonist |
| Evidence tracking, Experiment Assay: | Confocal Whole-cell patch clamp Optical physiology |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2872 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |