| Annotated protein: | Alpha-actinin-1 (Alpha-actinin cytoskeletal isoform) (F-actin cross-linking protein) (Non-muscle alpha-actinin-1). Gene symbol: ACTN1. Taxonomy: Homo sapiens (Human). Uniprot ID: P12814 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ACTN1 |
| Ontology domain: | Biological Process |
| SynGO term: | structural constituent of postsynapse (GO:0099186) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Matt L, et al. "alpha-Actinin Anchors PSD-95 at Postsynaptic Sites" Neuron. 2018 Mar 7;97(5):1094-1109.e9 PMID:29429936 |
| Figure(s): | Fig.1, 2, 6, 7, S2 |
| Annotation description: | Fig.1: "α-Actinin-1 CH1, CH2, and EF-Hand Domains Interact with PSD-95" Fig.2: "α-Actinin KD Reduces Synapse Density and Postsynaptic PSD-95 Content" Fig.6: "Molecular Replacement of α-Actinin with α-Actinin-1E53K or α-Actinin-1ED213,217KK and of PSD-95 with PSD-95K10E and PSD-95K11E Impairs Synaptic Localization of PSD-95 and AMPARs" Fig.7: "NMDA-Induced Ca2+ Influx Causes Disruption of the PSD-95-α-Actinin Interaction by Ca2+/CaM" Figure S2. "Knockdown of α-actinin reduces spine density without influencing the morphology of dendrites and spines" |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons Cell-free system |
| Evidence tracking, Protein Targeting: | RNAi / shRNA Over-expression |
| Evidence tracking, Experiment Assay: | Confocal IP + WB/MSMS |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2870 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |