| Annotated protein: | NAD-dependent protein deacetylase sirtuin-2 (EC 2.3.1.286) (NAD-dependent protein defatty-acylase sirtuin-2) (EC 2.3.1.-) (Regulatory protein SIR2 homolog 2) (SIR2-like protein 2). Gene symbol: SIRT2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q5RJQ4 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SIRT2 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynaptic neurotransmitter receptor endocytosis (GO:0099149) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Wang G, et al. "Crucial Roles for SIRT2 and AMPA Receptor Acetylation in Synaptic Plasticity and Memory" Cell Rep. 2017 Aug 8;20(6):1335-1347 PMID:28793258 |
| Figure(s): | Fig.4, 5, 6 |
| Annotation description: | Fig.4: "Acetylation regulates AMPAR trafficking, turnover and accumulation in neuron" - " To test whether acetylation affects AMPAR trafficking, we performed AMPAR internalization and insertion assays in cultured hippocampal neurons. We found that SIRT2 inhibition by B2 (20 μM, 24hrs) resulted in a suppression of agonist-induced (AMPA, 100 μM) AMPAR internalization (Beattie et al., 2000) (Figure 4A, n ≥ 15 cells/group, p < 0.05) without affecting glycine (200 μM)-induced receptor insertion (Lu et al., 2001) (Figure 4B, n ≥ 15 cells/group, p > 0.05)." Fig.5: "SIRT2 knock-out mice show enhanced AMPAR acetylation and impaired synaptic plasticity" Fig.6: "Sirt2-/- mice show impairments in learning and memory" |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) Antagonist / agonist |
| Evidence tracking, Experiment Assay: | Confocal Whole-cell patch clamp Field recordings Western blot |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2869 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |