| Annotated protein: | Ubiquitin carboxyl-terminal hydrolase 46 (EC 3.4.19.12) (Deubiquitinating enzyme 46) (Ubiquitin thioesterase 46) (Ubiquitin-specific-processing protease 46). Gene symbol: USP46. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: F1M625 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ USP46 |
| Ontology domain: | Cellular Component |
| SynGO term: | synapse (GO:0045202) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Huo Y, et al. "The deubiquitinating enzyme USP46 regulates AMPA receptor ubiquitination and trafficking" J Neurochem. 2015 Sep;134(6):1067-80 PMID:26077708 |
| Figure(s): | Fig.1 |
| Annotation description: | Fig.1: "UPS46 synaptic distribution and developmental expression" - " In 2-week-old cultured rat hippocampal neurons, USP46 was labeled with specific antibodies together with AMPAR GluA1 subunits, or with a postsynaptic marker protein PSD-95. USP46 showed a punctate distribution pattern and was mostly co-localized with GluA1 or PSD95, indicating synaptic localization (Fig. 1a and b)." - "Consistent with immunostaining results, western blots revealed that, similar to the known synaptic proteins including GluA1 and PSD95, USP46 was enriched in the synaptosomal P2 fractions compared with the lysates (Fig. 1c)." |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Wide-field fluorescence Western blot Biochemical fractionation (generic) |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2868 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |