| Annotated protein: | Ras-related protein Rab-5A (EC 3.6.5.2) (Small GTP-binding protein rab5). Gene symbol: RAB5A. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: M0RC99 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ RAB5A |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic endocytic zone membrane (GO:0098844) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Brown TC, et al. "NMDA receptor-dependent activation of the small GTPase Rab5 drives the removal of synaptic AMPA receptors during hippocampal LTD" Neuron. 2005 Jan 6;45(1):81-94 PMID:15629704 |
| Figure(s): | Fig.1 |
| Annotation description: | Fig.1: "Ultrastructural localization of endogenous Rab5 in CA1 neurons of rat hippocampus." - " Most synaptic Rab5 immunolabeling was found in the postsynaptic terminal (~75%; Figure 1A, arrows; Figure 1B, "POST"). Furthermore, we found that postsynaptic Rab5 labeling was predominantly located outside of the postsynaptic density (PSD) on lateral extrasynaptic membranes (Figure 1B, "Memb."), roughly 100-300 nm away from the edge of the PSD (Figure 1C). This localization fits well with "endocytic hotspots" previously described lateral to the PSD (Blanpied et al., 2002; Petralia et al., 2003; Racz et al., 2004)." Note: the imaging data by itself does not resolve the endocytic zone. The rest of the paper shows a role of Rab5 in AMPAR endocytosis and the localization lateral of the PSD is suggestive of the postsynaptic endocytic zone. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Confocal |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2861 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |