| Annotated protein: | Serine/threonine kinase 38. Gene symbol: STK38. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: G3V9Q4 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ STK38 |
| Ontology domain: | Biological Process |
| SynGO term: | postsynapse organization (GO:0099173) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Ultanir SK, et al. "Chemical genetic identification of NDR1/2 kinase substrates AAK1 and Rabin8 Uncovers their roles in dendrite arborization and spine development" Neuron. 2012 Mar 22;73(6):1127-42 PMID:22445341 |
| Figure(s): | Fig.3, 4 |
| Annotation description: | Fig.3: "NDR1/2's role on dendritic spine and excitatory postsynaptic development" - "Expression of dominant negative NDR1 (NDR1-KD or NDR1-AA) caused a robust increase of filopodia and atypical protrusion density together with a reduction in mushroom spine density (Figure 3A-C), indicating that NDR1 function is necessary for mushroom spine formation. In contrast, NDR1-CA drastically reduced the total dendritic protrusion density, as a result of significant reduction in mushroom spines, filopodia and stubby spines (Figure 3A-C). " - "Similar to the dominant negative effects of NDR1 mutants, NDR1siRNA + NDR2siRNA also resulted in increased filopodia and atypical protrusions and decreased mushroom spine densities, which was rescued by co-expression of siRNA resistant NDR1 (NDR1*) (Figure 3A & D)" Fig.4: "In vivo analysis of NDR1 mutants and siRNA by in utero electroporation" - " We found that NDR1-KD and NDR1siRNA + NDR2siRNA decreased dendritic spine head diameter (Figure 4G), suggesting that NDR1/2 is required for dendritic spine development in vivo" |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA Over-expression |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2856 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |