| Annotated protein: | Palmitoyltransferase ZDHHC5 (EC 2.3.1.225) (Zinc finger DHHC domain-containing protein 5) (DHHC-5). Gene symbol: ZDHHC5. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q2THW7 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ZDHHC5 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic specialization, intracellular component (GO:0099091) |
| Synapse type(s): | hippocampus, glutamatergic hippocampus, GABAergic |
| Annotated paper: | Brigidi GS, et al. "Activity-regulated trafficking of the palmitoyl-acyl transferase DHHC5" Nat Commun. 2015 Sep 3;6:8200 PMID:26334723 |
| Figure(s): | Fig.1, 2, 4 |
| Annotation description: | Fig.1: "Changes in DHHC5 palmitoylation and localization following increased neuronal activity." - "We next examined whether neuronal activity controls the palmitoylation of substrates by modifying the subcellular localization of DHHC enzymes. Under basal conditions, 56.9±5.8% of endogenous DHHC5 co-localized with the postsynaptic protein PSD-95 (Fig. 1c,f, n=14 cells), a faithful marker of excitatory synapses (Supplementary Fig. 1a,b). DHHC5 (31.18±2.53%) co-localized with the inhibitory postsynaptic protein gephyrin (Fig. 1h, n=32 cells), a faithful marker of inhibitory synapses (Supplementary Fig. 1c,d). Together, this indicates that ~88% of DHHC5 is localized to synapses. Furthermore, 79.73±4.92% of excitatory synapses and 46.78±1.77% of inhibitory synapses co-localize with DHHC5, indicating that the majority of synapses contain DHHC5." Fig.2: "Activity enhances DHHC5 trafficking from spines" - "GFP-DHHC5 puncta were localized to spine heads before cLTP treatment (now indicated in the figures as the absence or the presence of glycine treatment; ±Gly), translocated out of spines 2-3 min after stimulation and then trafficked back into spines 4 min after stimulation with significantly more DHHC5 localized to spines 5-20 min after stimulation (Fig. 2b,c)." Fig.4: "Activity regulates the association of DHHC5 with δ-catenin and PSD-95" - "The activity-induced increase in DHHC5-δ-catenin interactions (5-15 min, Fig. 4d) corresponded with a decrease in DHHC5-PSD-95 interactions (3-5 min, Fig. 4c,d) and coincided with the endocytosis (3 min, Fig. 3a,b) and trafficking of DHHC5 out of spines (2-5 min, Figs 2b-g,3a,b,d-f)." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal IP + WB/MSMS |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2854 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |