| Annotated protein: | G protein-activated inward rectifier potassium channel 2 (GIRK-2) (Inward rectifier K(+) channel Kir3.2) (Potassium channel, inwardly rectifying subfamily J member 6). Gene symbol: KCNJ6. Taxonomy: Mus musculus (Mouse). Uniprot ID: P48542 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ KCNJ6 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of presynaptic membrane (GO:0099056) |
| Synapse type(s): | Parallel fibre (cerebellum -> Purkinje-cell) |
| Annotated paper: | Fernandez-Alacid L, et al. "Subcellular compartment-specific molecular diversity of pre- and post-synaptic GABA-activated GIRK channels in Purkinje cells" J Neurochem. 2009 Aug;110(4):1363-76 PMID:19558451 |
| Figure(s): | Fig. 6a-d |
| Annotation description: | p.1371: (a) (b)(c) (d)Fig. 6 GABAB receptors co-localize with GIRK subunits in parallel fibre terminals. Electron micrographs show co-localization of GABAB1 and GIRK3 (a and b), as well as co-localization of GIRK1 or GIRK2 with GIRK3 (c and d), in the same parallel fibre terminal, as revealed using double-labelling pre-embedding methods. (a and b) The peroxidase reaction product (HRP) indicating GIRK3 immunoreactivity filled parallel fibre terminals (pf), whereas immunoparticles (GABAB1 immunoreactivity) were mainly located along the extrasynaptic plasma membrane, and occasionally in the pre-synaptic membrane specialization of parallel fibre terminals (pf) establishing excitatory synapses on PC spines (s) (arrows). (c and d) |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Niels Cornelisse (ORCID:0000-0001-9425-2935) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 285 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |