| Annotated protein: | Triple functional domain protein (EC 2.7.11.1) (PTPRF-interacting protein). Gene symbol: TRIO. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: F1M0Z1 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ TRIO |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Terry-Lorenzo RT, et al. "Trio, a Rho Family GEF, Interacts with the Presynaptic Active Zone Proteins Piccolo and Bassoon" PLoS One. 2016 Dec 1;11(12):e0167535 PMID:27907191 |
| Figure(s): | fig 1c, 2, 3 |
| Annotation description: | From Results section: "Similar to PSD-95, the CAZ proteins Piccolo, Bassoon, and ELKS2 were enriched in the synaptic junctional preparations (Fig 1C). Intriguingly, the FL, 9L, and 9S isoforms of Trio were also highly enriched in these preparations (Fig 1C). These results suggest that, while most Trio isoforms were down regulated during brain development (Fig 1B), the high molecular weight Trio isoforms (FL, 9S, 9L) that continue to be expressed in the adult brain were highly enriched in synaptic junctions (Fig 1C)." "Qualitatively, most Trio puncta were observed to co-localize with Piccolo and PSD-95 positive puncta (arrows in Fig 2B). In a quantitative assessment, we found that ~75% of PSD-95-positive Piccolo puncta were Trio positive, while less than 50% of PSD-95-negative Piccolo puncta were Trio positive (Fig 2D, left panel)." (...) "Similar results were obtained when the experiment was performed with antibodies to Synaptophysin and PSD-95 (Fig 2C and 2D), supporting the concept that a significant fraction of the longer Trio isoforms are localized at synapses in mature neurons." "In this analysis, GFP-Trio-FL exhibited a punctate pattern in the cell soma and dendrites, yet appeared excluded from the nucleus (Fig 3A). At higher magnification, GFP-Trio-FL clearly localized to dendritic shafts and spines (arrowhead in panel B)(Fig 3A and 3B). It was also present in axons (Fig 3A and 3C)" (...) "In the distal axon, GFP-Trio-FL was found in distinct puncta that frequently co-localized with Piccolo adjacent to the MAP2-labeled dendrites of untransfected neurons (arrows in Fig 3C). This analysis demonstrated that Trio, when expressed exogenously in mature cultured neurons, not only localized within dendritic spines, but also localized within presynaptic terminals" |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Western blot Biochemical fractionation (generic) |
| Annotator(s): | Rita Reig-Viader (ORCID:0000-0002-6893-6177) Àlex Bayés (ORCID:0000-0002-5265-6306) |
| Lab: | Molecular Physiology of the Synapse Laboratory, Biomedical Research Institute Sant Pau, 08025 Barcelona, Spain and and Universitat Autnoma de Cerdanyola del Valls, Spain Barcelona, 08193 Bellaterra |
| SynGO annotation ID: | 2849 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |