| Annotated protein: | G-protein coupled estrogen receptor 1 (G protein-coupled estrogen receptor 1) (G-protein coupled receptor 30). Gene symbol: GPER1. Taxonomy: Macaca Mulatta (Rhesus Macaque). Uniprot ID: F7EQ49 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ GPER1 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of presynaptic membrane (GO:0099056) |
| Synapse type(s): | neocortex, glutamatergic |
| Annotated paper: | Crimins JL, et al. "Diverse Synaptic Distributions of G Protein-coupled Estrogen Receptor 1 in Monkey Prefrontal Cortex with Aging and Menopause" Cereb Cortex. 2017 Mar 1;27(3):2022-2033 PMID:26941383 |
| Figure(s): | Fig.3, 4 |
| Annotation description: | Fig.3: "Densities of GPER1 immunogold particles in the presynaptic and postsynaptic compartments" Fig.4: "Subcellular synaptic distribution of GPER1 immunogold particles" - "GPER1 was also highly enriched in the plasmalemmal domains of both terminals and spines, as well as in the cytoplasmic and synaptic (comprised of both the PSD and bordering perisynaptic region) domains of spines. " Discussion: "The localization of GPER1 to dendritic spines, and to axon terminals where it was markedly abundant, is consistent with studies in rodent forebrain (Akama et al. 2013; Almey et al. 2014; Waters et al. 2015). Within these compartments, GPER1 was distributed to the plasmalemmal and cytoplasmic domains; to the postsynaptic density and its subjacent subsynaptic zone; and, to the active zone and presynaptic mitochondria. Many cytoplasmic GPER1 gold particles were associated with membranous structures, such as synaptic vesicles in the axon terminals and the spine apparatus postsynaptically. Such highly diversified subcellular localizations suggest that GPER1 mediates the complex activities of estrogen within the synapse, as well as other spine and terminal domains." - Based on the known transmembrane topology of the protein, the "integral component of ... membrane" term was chosen. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | Similar findings in mouse hippocampus (EM-data) @ PMID:25673833 |
| SynGO annotation ID: | 2846 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |