| Annotated protein: | MAM domain-containing glycosylphosphatidylinositol anchor protein 1 (GPI and MAM protein) (GPIM) (Glycosylphosphatidylinositol-MAM) (MAM domain-containing protein 3). Gene symbol: MDGA1. Taxonomy: Homo sapiens (Human). Uniprot ID: Q8NFP4 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ MDGA1 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of synaptic membrane adhesion (GO:0099179) |
| Synapse type(s): | hippocampus, GABAergic |
| Annotated paper: | Gangwar SP, et al. "Molecular Mechanism of MDGA1: Regulation of Neuroligin 2:Neurexin Trans-synaptic Bridges" Neuron. 2017 Jun 21;94(6):1132-1141.e4 PMID:28641112 |
| Figure(s): | Fig.1, 3, 4, 5 |
| Annotation description: | Fig.1: "Analysis of MDGA1-NL interaction using cell-surface binding assays" Fig.3: "MDGA knockdown increases inhibitory synapse density in an NL2-dependent manner in cultured neurons" Fig.4: "MDGA1 overexpression decreases inhibitory, but not excitatory, synaptic transmission in cultured cortical neurons" Fig.5: "MDGA1 cis expression inhibits NL2 activity in artificial synapse-formation assays and cell-adhesion assays" Coclusion: MDGA1 is a cis-interactor with NL2 and negatively affects its transsynaptic adhesion to neurexin and its ability to induce presynapse assembly. |
| Evidence tracking, Biological System: | Cultured neurons Non-neuronal tissue |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Confocal Whole-cell patch clamp |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2840 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |