| Annotated protein: | Regulator of G-protein signaling 7 (RGS7). Gene symbol: RGS7. Taxonomy: Mus musculus (Mouse). Uniprot ID: O54829 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ RGS7 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynaptic membrane potential (GO:0060078) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Ostrovskaya O, et al. "RGS7/Gbeta5/R7BP complex regulates synaptic plasticity and memory by modulating hippocampal GABABR-GIRK signaling" Elife. 2014 Apr 22;3:e02053 PMID:24755289 |
| Figure(s): | Fig.1-3, 5, 6 |
| Annotation description: | Fig.1: "RGS7 and R7BP modulate GABABR-GIRK signaling in cultured hippocampal neurons" Fig.2: "Timing and sensitivity of GABABR-GIRK signaling is differentially controlled by RGS7 and R7BP" Fig.3: "Changing the ratio of Gβγ to an effector affects response deactivation lag in a reconstituted system" [Fig.4: "Change in subcellular localization of RGS7 in the hippocampus of the R7bp-/- mice"] Fig.5: "Altered intrinsic excitability and excitatory transmission in CA1 hippocampal neurons from Rgs7-/- mice" Fig.6: "RGS7 ablation disrupts hippocampal synaptic plasticity" [Fig.7: "RGS ablation affects hippocampal-dependent learning and memory"] Summary: - RGS7 and R7BP modulate the activity of GABABR and regulate its signaling via G-proteins to the G-protein activated ion channel GRIK. This in turn influences the postsynaptic excytability and ultimately influences LTP induction. The site of action for RGS7 and R7BP is postsynaptic as concluded by the authors: "There were no significant differences in either amplitudes or frequencies of sEPSC events between Rgs7-/- and wild-type neurons (Figure 5F-G). These observations indicate that excitatory input into CA1 pyramidal neurons is unchanged by elimination of RGS7 and suggest that the observed decrease in neuronal excitability is caused by changes in the intrinsic membrane properties of CA1 neurons, likely stemming from enhanced activity of the postsynaptic GIRK channel." - The mechanism by which RGS7 and R7BP reglate the activity is via the clearance of active G-proteins that were produced by the activated GABABR. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons Non-neuronal tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Whole-cell patch clamp |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2836 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |