| Annotated protein: | Potassium voltage-gated channel subfamily Q member 5. Gene symbol: KCNQ5. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: F1LY25 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ KCNQ5 |
| Ontology domain: | Biological Process |
| SynGO term: | voltage-gated ion channel activity involved in regulation of presynaptic membrane potential (GO:0099508) |
| Synapse type(s): | Calyx of Held |
| Annotated paper: | Huang H, et al. "KCNQ5 channels control resting properties and release probability of a synapse" Nat Neurosci. 2011 Jun 12;14(7):840-7 PMID:21666672 |
| Figure(s): | Fig. 1a-c, Fig. 3K-L |
| Annotation description: | Figure 1: Identification of presynaptic KCNQ current: (a) A slow voltage ramp (13 mV s-1) with a K+-based internal solution evoked an outward current (black) that was strongly inhibited by 10-20 μM XE991, a KCNQ channel blocker (gray). (b) Outward current blocked by the KCNQ channel blocker linopirdine. (c) Outward current potentiated by the KCNQ channel opener flupirtine. Figure 3: Evidence for the presynaptic KCNQ subunit to be KCNQ5: Given the very low expression of KCNQ2-KCNQ4, these results suggest that XE991-sensitive current is generated by channels composed solely of KCNQ5 subunits. The conclusions based on immunohistochemistry were con- firmed by a pharmacological approach. Recent studies have iden- tified compounds whose effects differ qualitatively among different KCNQ subunits. Presynaptic K+ current was measured under con- ditions in which almost all current is XE991 sensitive (Fig. 1a). Diclofenac is a KCNQ2-KCNQ4 activator but a blocker of KCNQ5 (ref. 26). We found that 100 μM diclofenac inhibited the presyn- apticK+ currentby53±4%at0mV(Fig.3k;P=0.004,n=5). Among cloned KCNQ subunits, UCL2077 is reported to inhibit all but KCNQ5, which exhibits voltage dependent potentiation by the drug27. UCL2077 at 10 μM potentiated K+ current by 8 ± 2% at 0 mV (Fig. 3l; P = 0.05, n = 4). Although it is controversial whether KCNQ1 is present in the CNS28, this subunit does not have a pro- file of sensitivity to diclofenac or UCL2077 resembling that of the presynaptic current26,27. These results support our immunohisto- chemical observations and indicate that the presynaptic KCNQ subunit is likely to be KCNQ5. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antagonist / agonist |
| Evidence tracking, Experiment Assay: | Whole-cell patch clamp |
| Annotator(s): | Niels Cornelisse (ORCID:0000-0001-9425-2935) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 283 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |