| Annotated protein: | Calsyntenin-1 (Alcadein-alpha) (Alc-alpha) [Cleaved into: Soluble Alc-alpha (SAlc-alpha); CTF1-alpha (C-terminal fragment 1-alpha)]. Gene symbol: CLSTN1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q9EPL2 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CLSTN1 |
| Ontology domain: | Biological Process |
| SynGO term: | neurotransmitter receptor transport to postsynaptic membrane (GO:0098969) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Ster J, et al. "Calsyntenin-1 regulates targeting of dendritic NMDA receptors and dendritic spine maturation in CA1 hippocampal pyramidal cells during postnatal development" J Neurosci. 2014 Jun 25;34(26):8716-27 PMID:24966372 |
| Figure(s): | Fig.3, 5-8 |
| Annotation description: | Fig.3: "Excitatory synaptic transmission is reduced in juvenile Cst-1 KO mice" Figure 5: "Calsyntenin-1 colocalizes with dendritic endosomes that transport NMDA receptor subunits" - "the considerable degree of colocalization of dendritic calsyntenin-1 puncta with both the transferrin receptor (TfR), a marker for recycling endosomes, as well as with APP, which is carried in early endosomal calsyntenin-1 vesicles (Steuble et al., 2012), indicates that dendritic calsyntenin-1-associated vesicles are involved in transport of both early and recycling endosomal organelles (Fig. 5A,B). Importantly, dendritic calsyntenin-1 also colocalizes with NMDA receptor subunits (Fig. 5C,D)." Figure 6: "Levels of GluN2B subunit-containing NMDA receptors are increased in juvenile Cst-1 KO mice" Figure 7: "Enhanced NMDA receptor-dependent LTP at Schaffer collateral-CA1 pyramidal cell synapses in juvenile Cst-1 KO mice" Figure 8: "The spine-type distribution is altered in CA1 pyramidal cells from juvenile Cst-1 KO mice" Note: the transport defect proposed by the authors is considered the primary function of the protein in this paper. The downstream functional consequence are not annotated separately. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Whole-cell patch clamp Field recordings Western blot |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2828 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |