| Annotated protein: | Calsyntenin-1 [Cleaved into: Soluble Alc-alpha (SAlc-alpha); CTF1-alpha (C-terminal fragment 1-alpha)]. Gene symbol: CLSTN1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q6Q0N0 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CLSTN1 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic density membrane (GO:0099061) |
| Synapse type(s): | hippocampus, glutamatergic hippocampus, GABAergic |
| Annotated paper: | Vogt L, et al. "Calsyntenin-1, a proteolytically processed postsynaptic membrane protein with a cytoplasmic calcium-binding domain" Mol Cell Neurosci. 2001 Jan;17(1):151-66 PMID:11161476 |
| Figure(s): | Fig.6, 7 |
| Annotation description: | Fig.6: "Ultrastructural localization of calsyntenin-1 in the postsynaptic membrane of spine synapses." - "Postembedding immunogold labeling of rat hippocampus embedded at low temperature confirmed the localization of calsyntenin-1 in the postsynaptic membrane (Figs. 6D-6E). Both asymmetric synapses with round vesicles and thick PSDs (Type 1 according to Gray (1959)) and a subpopulation of symmetric synapses with pleomorphic vesicles and thin PSDs (Type 2) exhibited calsyntenin-1 immunoreactivity, confirming calsyntenin-1 as a component of the postsynaptic membrane in both excitatory and inhibitory synapses" Fig.7: "Subcellular fractionation localizes calsyntenin-1 in synaptosomes, but not in postsynaptic densities" - "As demonstrated in Fig. 7, synaptosomes were enriched in full-length calsyntenin-1 and its cleavage products. Hypotonic disruption of synaptosomes and treatment with a mild detergent resulted in the solubilization of all three forms of calsyntenin-1. In contrast, typical markers of the postsynaptic density, viz. PSD-95 and GluR1 (O'Brien et al., 1998), remained in the particulate fractions (P4 and PSD, according to Phelan and GordonWeeks, 1997). The clearance of calsyntenin-1 from the PSD fraction indicates that its cytoplasmic segment is not firmly associated with the subsynaptic molecular scaffold that corresponds to the PSD observed in the EM and that is operationally defined as the particulate matter resulting after detergent treatment of synaptosomes." |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Western blot Biochemical fractionation (generic) |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2826 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |