| Annotated protein: | Potassium voltage-gated channel subfamily Q member 5. Gene symbol: KCNQ5. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: F1LY25 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ KCNQ5 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of presynaptic membrane (GO:0099056) |
| Synapse type(s): | Calyx of Held |
| Annotated paper: | Caminos E, et al. "The potassium channel KCNQ5/Kv7.5 is localized in synaptic endings of auditory brainstem nuclei of the rat" J Comp Neurol. 2007 Dec 1;505(4):363-78 PMID:17912742 |
| Figure(s): | Fig. 1F-G, Fig. 5A-H, Fig. 7H,I |
| Annotation description: | Fig 1.: immunolabeling of bouton-like structures: High-magnification pictures of cell bodies encrusted with intensely immunolabeled bouton-like struc- tures (arrows). Spherical cells in F from the boxed area in E; globular cells and multipolar cells (arrow) with labeled boutons around somata and proximal dendrites (arrowhead) in G; and neurons in the auditory nerve root with immunoreactive boutons around cell bodies and den- drites in H. Fig 5. Colocalization of KCNQ5 with presynaptic markers (synaptophysin and syntax): Merged confocal images of double fluorescence immunola- beling for KCNQ5 (green color) and markers for synaptic endings (syntaxin in A-D and synaptophysin in E-H, magenta color) in coro- nal sections through auditory brainstem nuclei. Arrows highlight colocalization of both proteins (white color) in neurons from the co- chlear nerve root (A), the anteroventral cochlear nucleus (AVCN; B), the lateral superior olive (LSO; D), the medial nucleus of the trape- zoid body (MNTB; E), the medial superior olive (MSO; F), nuclei of lateral lemniscus (NLL; G), and the central nucleus of the inferior nerve terminates (Rhode et al., 1983; Blackstad et al., 1984; Moore, 1986). In the deep layer of the DCN, KCNQ5 immunoreactivity was found in abundant ending-like structures around cell bodies and proximal dendrites of giant cells, which also are targets of auditory nerve fibers. colliculus (CNIC; H). Fig 7: Los of KCNQ5 staining after ablation of presynaptic fibers: In a different set of experiments, we investigated whether KCNQ5 was present in excitatory endings of the auditory nerve. This was observed in rats cochleotomized at 30 days old (Fig. 7G-I). The degree of cochlear nerve disintegration at its entrance to the CN was evaluated in sections labeled with an anti-calretinin antibody. Most calretinin labeling in fibers had disappeared from the neuropil 10 days after cochlear ablation, indicating exten- sive fiber and terminal degeneration in the CN (Illing et al., 1997; Caminos et al., 2005). In sections from cochleoto- mized animals labeled with immunoperoxidase to detect KCNQ5 immunoreactivity, it was seen that auditory nerve fiber degeneration was matched by a dramatic de- crease of KCNQ5-labeled endings around cell bodies and in the neuropil (Fig. 7H,I). Often, KCNQ5 immunoreac- tivity completely disappeared, which was particularly well seen in synaptic endings around cell bodies in the AVCN (Fig. 7I). Therefore, excitatory endings from the auditory nerve in the AVCN are a main source of synaptic endings where KCNQ5 is expressed. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Microscopy (generic) |
| Annotator(s): | Niels Cornelisse (ORCID:0000-0001-9425-2935) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 282 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |