Annotated protein:Carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein (C-terminal PDZ ligand of neuronal nitric oxide synthase protein) (Nitric oxide synthase 1 adaptor protein). Gene symbol: NOS1AP. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: O54960
antibody wiki:
SynGO gene info:SynGO data @ NOS1AP
Ontology domain:Cellular Component
SynGO term:presynapse (GO:0098793)
Synapse type(s):hippocampus, glutamatergic
Annotated paper:Richier L, et al. "NOS1AP associates with Scribble and regulates dendritic spine development" J Neurosci. 2010 Mar 31;30(13):4796-805 PMID:20357130
Figure(s):Fig.4
Annotation description:Fig.4: "NOS1AP and Scrib colocalize to synaptophysin-positive presynaptic specializations in axons."
- "Together with our results showing that Scrib associates with presynaptic proteins, these localization data suggest that NOS1AP localizes with Scrib to presynaptic specializations, where it may play a role in synaptic vesicle dynamics and active zone formation."

[Fig.5: "NOS1AP and Scrib colocalize in dendrites"
- "We cotransfected dissociated rat hippocampal neurons with constructs encoding YFP-NOS1AP, CFP-Scrib, and RFP-synaptophysin. We saw colocalization of all three markers, supporting the notion that NOS1AP and Scrib could play a role presynaptically (Fig. 4G-J, arrows). This analysis showed colocalization of YFP-NOS1AP and actin in spine heads (Fig. 5K-M, arrows), supporting the notion that NOS1AP is found in dendritic spines. Together, these data suggest that NOS1AP and Scrib proteins can localize postsynaptically in dendritic spines."]
Evidence tracking, Biological System:Cultured neurons
Evidence tracking, Protein Targeting:Over-expression
Evidence tracking, Experiment Assay:Confocal
Annotator(s):Pim van Nierop (ORCID:0000-0003-0593-3443)
Guus Smit (ORCID:0000-0002-2286-1587)
Matthijs Verhage (ORCID:0000-0002-2514-0216)
Lab:Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands
SynGO annotation ID:2819
Dataset release (version):20231201
View annotation as GO-CAM model:Gene Ontology