| Annotated protein: | Carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein (C-terminal PDZ ligand of neuronal nitric oxide synthase protein) (Nitric oxide synthase 1 adaptor protein). Gene symbol: NOS1AP. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: O54960 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ NOS1AP |
| Ontology domain: | Cellular Component |
| SynGO term: | presynapse (GO:0098793) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Richier L, et al. "NOS1AP associates with Scribble and regulates dendritic spine development" J Neurosci. 2010 Mar 31;30(13):4796-805 PMID:20357130 |
| Figure(s): | Fig.4 |
| Annotation description: | Fig.4: "NOS1AP and Scrib colocalize to synaptophysin-positive presynaptic specializations in axons." - "Together with our results showing that Scrib associates with presynaptic proteins, these localization data suggest that NOS1AP localizes with Scrib to presynaptic specializations, where it may play a role in synaptic vesicle dynamics and active zone formation." [Fig.5: "NOS1AP and Scrib colocalize in dendrites" - "We cotransfected dissociated rat hippocampal neurons with constructs encoding YFP-NOS1AP, CFP-Scrib, and RFP-synaptophysin. We saw colocalization of all three markers, supporting the notion that NOS1AP and Scrib could play a role presynaptically (Fig. 4G-J, arrows). This analysis showed colocalization of YFP-NOS1AP and actin in spine heads (Fig. 5K-M, arrows), supporting the notion that NOS1AP is found in dendritic spines. Together, these data suggest that NOS1AP and Scrib proteins can localize postsynaptically in dendritic spines."] |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2819 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |