| Annotated protein: | Carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein (C-terminal PDZ ligand of neuronal nitric oxide synthase protein) (Nitric oxide synthase 1 adaptor protein). Gene symbol: NOS1AP. Taxonomy: Homo sapiens (Human). Uniprot ID: O75052 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ NOS1AP |
| Ontology domain: | Biological Process |
| SynGO term: | postsynaptic actin cytoskeleton organization (GO:0098974) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Hernandez K, et al. "Overexpression of Isoforms of Nitric Oxide Synthase 1 Adaptor Protein, Encoded by a Risk Gene for Schizophrenia, Alters Actin Dynamics and Synaptic Function" Front Cell Neurosci. 2016 Feb 2;10:6 PMID:26869880 |
| Figure(s): | Fig.1, 2, 4, 5, 6, 7 |
| Annotation description: | Fig.1: "Expression of NOS1AP-L or NOS1AP-S in COS-7 cells decreases F-actin" Fig.2: "Effects of NOS1AP-L or NOS1AP-S overexpression on protrusion index and F-actin" Fig.3: "NOS1AP-S decreases total Rac1 and cofilin protein expression in HEK293T cells" Fig.4: "NOS1AP-L and NOS1AP-S associate with F-actin and promote actin polymerization" Fig.5: "NOS1AP-L alter spine number and morphology." Fig.6: "NOS1AP-S alter spine number and morphology" Fig.7: "NOS1AP-S increases synaptic strength, while NOS1AP-L decreases synaptic strength" |
| Evidence tracking, Biological System: | Cultured neurons Non-neuronal tissue |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Confocal Whole-cell patch clamp Western blot |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | Fig.6: "NOS1AP increases processes outgrowth and influences Rac activity" - "For this purpose, we overexpressed either YFP or YFP-NOS1AP in dissociated hippocampal neurons that had been cultured for 13 DIV (Fig. 6A,B). Twenty-four hours after transfection, we detected a 30% increase in the number of dendritic protrusions in the YFP-NOS1AP-transfected neurons (Fig. 6E)." @ PMID:20357130 |
| SynGO annotation ID: | 2818 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |