| Annotated protein: | Protein Wnt-7a. Gene symbol: WNT7A. Taxonomy: Homo sapiens (Human). Uniprot ID: O00755 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ WNT7A |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of presynapse assembly (GO:1905606) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Sahores M, et al. "Frizzled-5, a receptor for the synaptic organizer Wnt7a, regulates activity-mediated synaptogenesis" Development. 2010 Jul;137(13):2215-25 PMID:20530549 |
| Figure(s): | Fig.3, 4, 5 |
| Annotation description: | Fig. 3: "Fz5 stimulates the formation of presynaptic sites" - "Axonal Fz5 expression induces a 78% and a 36% increase in the number of synapsin I and bassoon puncta, respectively (Fig. 3C,D). Thus, expression of Fz5 in axons induces presynaptic differentiation in a similar manner to the synaptogenic factor Wnt7a." Fig.4: "Fz5 promotes synapse formation by binding to Wnt7a" - "Therefore, Fz5CRD completely blocks the synaptogenic activity of Wnt7a, suggesting that Fz5 mediates Wnt7a function at synapses." - "In hippocampal neurons, the Fz5 shRNA mixture significantly decreased the level of endogenous Fz5, whereas nc-shRNA had no effect (Fig. 5C)." Fig.5: "Fz5 knockdown abolishes Wnt7a-induced synapse formation" |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA Over-expression Antagonist / agonist |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2811 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |