| Annotated protein: | Frizzled-9 (Frizzled-like protein 9) (rFz9). Gene symbol: FZD9. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q8K4C8 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ FZD9 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Ramirez VT, et al. "Wnt-5a/Frizzled9 Receptor Signaling through the Galphao-Gbetagamma Complex Regulates Dendritic Spine Formation" J Biol Chem. 2016 Sep 2;291(36):19092-107 PMID:27402827 |
| Figure(s): | Fig.1 |
| Annotation description: | Fig.1: "FZD9 is located in the postsynaptic region." - "Our immunolocalization studies showed that FZD9 was distributed in a punctate pattern in the neuronal processes, as previously reported (31), and co-localized with the postsynaptic density scaffolding protein PSD-95 (Fig. 1A). This observation was supported by the Manders overlap coefficient, which represents the number of co-localized pixels expressed as a fraction of the total number of pixels in each channel. The co-localization index between PSD-95 and FZD9 was significantly higher than the co-localization between FZD9 and Piccolo, a presynaptic marker (Fig. 1B). Additionally, PSD-95 displays a higher co-localization index with FZD9 than those with FZD2, FZD3, or FZD7 and PSD-95, suggesting that this co-distribution was specific for FZD9 (Fig. 1C). The postsynaptic distribution of FZD9 was also analyzed biochemically in adult rat brains. Synaptosomal preparations were separated in the synaptophysin (SYP)-enriched (Triton X-100-soluble) fraction and the PSD-95-enriched (Triton X-100-insoluble) fraction (Fig. 1D). Consistent with the distribution observed in the cultured neurons, Western blotting showed that FZD9 was highly enriched in the PSD-95-containing fraction, whereas it was almost undetectable in the SYP-enriched fraction. Together, these data strongly suggest that FZD9 is located in the postsynaptic region of hippocampal neurons." |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Western blot Biochemical fractionation (generic) |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2806 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |