Annotated protein:Protein Wnt-5a. Gene symbol: WNT5A. Taxonomy: Homo sapiens (Human). Uniprot ID: P41221
antibody wiki:
SynGO gene info:SynGO data @ WNT5A
Ontology domain:Biological Process
SynGO term:regulation of postsynaptic cytosolic calcium levels (GO:0099566)
Synapse type(s):hippocampus, glutamatergic
Annotated paper:Ramirez VT, et al. "Wnt-5a/Frizzled9 Receptor Signaling through the Galphao-Gbetagamma Complex Regulates Dendritic Spine Formation" J Biol Chem. 2016 Sep 2;291(36):19092-107 PMID:27402827
Figure(s):Fig.2, 4
Annotation description:Fig.2: "CRD-FZD9 blocks the calcium increase produced by Wnt-5a"
- "Because Wnt-5a triggers an increase in intracellular Ca2+ concentration in hippocampal neurons (13), we attempted to understand the potential role of FZD9 in this effect by measuring the intracellular Ca2+ changes using the molecular probe Fura-2 AM. As expected, Wnt-5a treatment induced a rapid increase in the intensity of the Fura-2 AM signal (340/380 ratio), indicating a positive signaling modulation in intracellular Ca2+. Treatment with CRD-FZD9 blocked the Wnt-5a-mediated increase in the Ca2+ concentrations, whereas CRD-FZD9 has no effect (Fig. 2B). "

Fig.4: "FZD9 down-expression impairs Wnt-5a signaling"
- "These experiments reveal that down-regulation of endogenous FZD9 selectively impairs the Wnt-5a-induced rapid increase in intracellular Ca2+ concentrations in hippocampal neurons."
- "As shown in Fig. 4E, Wnt-5a treatment induced an increase in the number of dendritic protrusions in Fux-EGFP-transfected neurons, with a maximal effect at 2 h; however, this effect was reduced to basal levels in shFZD9-transfected neurons. "
Evidence tracking, Biological System:Cultured neurons
Evidence tracking, Protein Targeting:Over-expression
Evidence tracking, Experiment Assay:Confocal
Annotator(s):Pim van Nierop (ORCID:0000-0003-0593-3443)
Guus Smit (ORCID:0000-0002-2286-1587)
Matthijs Verhage (ORCID:0000-0002-2514-0216)
Lab:Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands
SynGO annotation ID:2804
Dataset release (version):20231201
View annotation as GO-CAM model:Gene Ontology