| Annotated protein: | Protein Wnt-5a. Gene symbol: WNT5A. Taxonomy: Homo sapiens (Human). Uniprot ID: P41221 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ WNT5A |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynapse organization (GO:0099175) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Ramirez VT, et al. "Wnt-5a/Frizzled9 Receptor Signaling through the Galphao-Gbetagamma Complex Regulates Dendritic Spine Formation" J Biol Chem. 2016 Sep 2;291(36):19092-107 PMID:27402827 |
| Figure(s): | Fig.3, 4 |
| Annotation description: | [Fig.2: "CRD-FZD9 blocks the calcium increase produced by Wnt-5a" - "Because Wnt-5a triggers an increase in intracellular Ca2+ concentration in hippocampal neurons (13), we attempted to understand the potential role of FZD9 in this effect by measuring the intracellular Ca2+ changes using the molecular probe Fura-2 AM. As expected, Wnt-5a treatment induced a rapid increase in the intensity of the Fura-2 AM signal (340/380 ratio), indicating a positive signaling modulation in intracellular Ca2+. Treatment with CRD-FZD9 blocked the Wnt-5a-mediated increase in the Ca2+ concentrations, whereas CRD-FZD9 has no effect (Fig. 2B). "] Fig.3: "CRD-FZD9 inhibits the spinogenesis triggered by Wnt-5a treatment" - "In agreement with previous reports (13), we observed that Wnt-5a increased the dendritic spine density (Fig. 3B). Interestingly, the exogenous addition of CRD-FZD9 inhibited the Wnt-5a-mediated increase in the density of dendritic protrusions after 2 h of Wnt-5a treatment." Fig.4: "FZD9 down-expression impairs Wnt-5a signaling" - "These experiments reveal that down-regulation of endogenous FZD9 selectively impairs the Wnt-5a-induced rapid increase in intracellular Ca2+ concentrations in hippocampal neurons." - "As shown in Fig. 4E, Wnt-5a treatment induced an increase in the number of dendritic protrusions in Fux-EGFP-transfected neurons, with a maximal effect at 2 h; however, this effect was reduced to basal levels in shFZD9-transfected neurons. " |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2803 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |