Annotated protein:receptor protein-tyrosine kinase (EC 2.7.10.1). Gene symbol: EPHB2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: A0A0G2JY48
antibody wiki:
SynGO gene info:SynGO data @ EPHB2
Ontology domain:Biological Process
SynGO term:postsynaptic membrane assembly (GO:0097104)
Synapse type(s):hippocampus, glutamatergic
Annotated paper:Dalva MB, et al. "EphB receptors interact with NMDA receptors and regulate excitatory synapse formation" Cell. 2000 Dec 8;103(6):945-56 PMID:11136979
Figure(s):Figs. 1, 2, 3, 4, 6, 7
Annotation description:Fig. 1 shows that EphB2 and NMDA subunit GluN1 co-associate into a physical complex after heterologous expression and in rat cortex. Activation of EphB2 by aggregated ephrinB-Fc induces interaction and co-clustering of EphB2 with GluN1 in cultured cortical neurons (Fig. 2, 3). Other NMDAR subunits (GluN2A and GluN2B) co-cluster with EphB2 as well (Fig .4).

Fig. 6 shows that treatment of cultured neurons with the EphB ligand EphrinB-Fc increases the number of NMDAR containing specializations, and the number of pre- and postsynaptic specializations in cultured neurons.

Fig. 7 shows that elevating EphB2 in cultured neurons increases the number of NMDAR clusters along dendrites.
Evidence tracking, Biological System:Intact tissue
Cultured neurons
Evidence tracking, Protein Targeting:Over-expression
Antibody (detection)
Evidence tracking, Experiment Assay:Confocal
Western blot
IP + WB/MSMS
Annotator(s):Tony Cijsouw (ORCID:0000-0003-0912-1514)
Thomas Biederer (ORCID:0000-0002-3670-7863)
Lab:Department of Neuroscience, Tufts University School of Medicine, Boston, MA 02111, USA
Additional literature:Fig. 2, EphB2 binds NR1.
Fig. 5, EphB2 regulates long-lasting LTP at Schaffer collateral synapses after theta burst stimulation.
Fig. 6, EphB2 regulates LTD at Schaffer collateral synapses after low frequency stimulation. @ PMID:11754835
SynGO annotation ID:280
Dataset release (version):20231201
View annotation as GO-CAM model:Gene Ontology