| Annotated protein: | Protein Wnt-5a. Gene symbol: WNT5A. Taxonomy: Homo sapiens (Human). Uniprot ID: P41221 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ WNT5A |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of synapse organization (GO:0050807) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Davis EK, et al. "Wnts acting through canonical and noncanonical signaling pathways exert opposite effects on hippocampal synapse formation" Neural Dev. 2008 Nov 5;3:32 PMID:18986540 |
| Figure(s): | Fig.8 |
| Annotation description: | Fig.8: "Wnt5a acts through a non-canonical pathway and decreases the number of presynaptic input" - "We exposed cultures to recombinant Wnt5a at 8 DIV for 36 hours. We found that Wnt5a decreases 'active' β-catenin immunofluorescence intensity, indicating that Wnt5a does not activate the canonical pathway and may act as an antagonist to canonical signaling (Figure 8A-D)." - "To determine if Wnt5a affects hippocampal synapse formation, we exposed cultures to Wnt5a at 8 DIV for 36 hours and observed the effect on the number of VGLUT1 puncta. In contrast to canonical Wnts, exposure to Wnt5a led to a significant decrease in the number of presynaptic terminals (Figure 8E, F), implying that the activation of a noncanonical pathway negatively regulates synapse formation." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2799 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |