| Annotated protein: | Protein Wnt-3a. Gene symbol: WNT3A. Taxonomy: Mus musculus (Mouse). Uniprot ID: P27467 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ WNT3A |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynapse to nucleus signaling pathway (GO:1905539) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Chen J, et al. "Activity-dependent synaptic Wnt release regulates hippocampal long term potentiation" J Biol Chem. 2006 Apr 28;281(17):11910-6 PMID:16501258 |
| Figure(s): | Fig.3 |
| Annotation description: | Fig.3: "Tetanus-induced nuclear accumulation of β-catenin" - "we tested the hypothesis that activity-induced Wnt release activates intracellular signaling pathways" - "Quantification of nuclear β-catenin signals of randomly selected DG granule cells showed that tetanus significantly increased β-catenin signals in the nuclei at 15 and 30 min after stimulations (Fig. 3, a-d)." - "We also found that APV completely abolished tetanus-induced nuclear accumulations of β-catenin (Fig. 3, e-h), indicating an essential role of NMDA receptors in tetanus-induced activation of the canonical Wnt/β-catenin pathway. In support of the activation of Wnt/β-catenin signaling after tetanic stimulations, we observed, based on a DNA microarray analysis,3 that tetanus up-regulated mRNAs of several Wnt target genes (Fig. 3m); these Wnt target genes included Dll1 (41, 42), Wisp-2 (43), Cldn1 (44), Fas (45), and CDD44 (46)." - "we investigated whether blockade of released Wnts can abolish tetanus-induced nuclear accumulation of β-catenin. To this end, we treated hippocampal slices with purified Frizzled-8-Fc (5 μg/ml), which is a Wnt scavenger (47). The results showed that Frizzled-8-Fc treatment abolished tetanus-induced nuclear accumulation of β-catenin (Fig. 3, i-l). \" |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antagonist / agonist |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2794 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |