| Annotated protein: | Protein Wnt-3a. Gene symbol: WNT3A. Taxonomy: Mus musculus (Mouse). Uniprot ID: P27467 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ WNT3A |
| Ontology domain: | Biological Process |
| SynGO term: | modulation of chemical synaptic transmission (GO:0050804) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Chen J, et al. "Activity-dependent synaptic Wnt release regulates hippocampal long term potentiation" J Biol Chem. 2006 Apr 28;281(17):11910-6 PMID:16501258 |
| Figure(s): | Fig.2, 4, 5 |
| Annotation description: | Fig.2: "Wnt3a release induced by tetanic stimulations" - "Tetanic stimulations also caused significant decreases of stimulated/non-stimulated ratios of the staining area (0.52 ± 0.06) and decreases of the number (0.74 ± 0.04) and the size (0.67 ± 0.05) of Wnt3a puncta (Fig. 2, k-m). These results strongly suggest that LTP-inducing tetanic stimulations led to a decrease of Wnt3a amount at synapses. Because the decrease of Wnt3a was observed immediately after tetanus, they were most likely due to the release of Wnt3a rather than other more time-consuming processes such as protein degradation." Fig.4: "Inhibition of Wnt signaling impaired LTP" - "To specifically antagonize Wnt3a, hippocampal slices were incubated for 2 h before LTP induction with anti-Wnt3a antibodies, which is known to neutralize Wnt3a activities in vitro (R & D Systems). Compared with purified nonspecific IgG controls, anti-Wnt3a antibodies (1 μg/ml) significantly impaired LTP (Fig. 4, c and d)." Fig.5: "Activation of Wnt signaling enhanced LTP" - "We found that, compared with control treatments without Wnt3A, pre-incubation of hippocampal slices with Wnt3a (200 ng/ml) potentiated LTP (Fig. 5c)." |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Over-expression Antagonist / agonist Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Field recordings |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | Similar observations in vivo @ PMID:28012021 |
| SynGO annotation ID: | 2793 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |