| Annotated protein: | Ras-related C3 botulinum toxin substrate 1 (EC 3.6.5.2) (p21-Rac1). Gene symbol: RAC1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q6RUV5 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ RAC1 |
| Ontology domain: | Biological Process |
| SynGO term: | postsynaptic actin cytoskeleton organization (GO:0098974) |
| Synapse type(s): | hippocampus, GABAergic |
| Annotated paper: | Smith KR, et al. "GIT1 and betaPIX are essential for GABA(A) receptor synaptic stability and inhibitory neurotransmission" Cell Rep. 2014 Oct 9;9(1):298-310 PMID:25284783 |
| Figure(s): | Fig.4 |
| Annotation description: | Fig.4: "Rac1 Is Localized at Inhibitory Synapses and Maintains Surface GABAAR Stability" - "Immunostaining and CLSM of hippocampal neurons transfected with GFP or Rac1-DN revealed a decrease in surface GABAAR cluster area and VGAT area in neurons expressing Rac1-DN compared with control neurons (Figures 4C-4E). In addition, surface biotinylation assays revealed that blocking Rac1 activation with the Rac1-DN caused a decrease in GABAAR surface levels (Figures 4F and 4G)." - "As an alternative approach, we determined if acute short-term inhibition of Rac1 would cause similar effects on GABAAR surface levels. To achieve this, we incubated neurons for 1 hr with the Rac1 inhibitor EHT 1864 (EHT; Shutes et al., 2007), followed by immunocytochemistry and CLSM. Similar to the results with Rac1-DN, analysis of these neurons showed that acute inhibition of Rac1 activity reduced the area of GABAAR and gephyrin clusters (Figures 4H-4J). " The effect of Rac1 on F-actin is not shown directly, but follows from 1) known role of Rac1 in actin organization, and the experiments on GIT1 and Rac1-GEF betaPix. Effect on actin and GABAR localization by GIT1 is shown in Fig.2. 20/01/2021 Dnyanada: Q6RUV5 (RAC1_RAT) was chosen on the basis of the biological system in which the experiment was conducted as the species of the overexpressed Rac1-DN could not be retrieved. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression Antagonist / agonist |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2791 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |