| Annotated protein: | Ephrin-B2 (ELF-2) (EPH-related receptor tyrosine kinase ligand 5) (LERK-5) (HTK ligand) (HTK-L). Gene symbol: EFNB2. Taxonomy: Mus musculus (Mouse). Uniprot ID: P52800 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ EFNB2 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynaptic membrane neurotransmitter receptor levels (GO:0099072) |
| Synapse type(s): | hippocampus, glutamatergic Schaffer collateral synapse (CA3->CA1) |
| Annotated paper: | Henderson JT, et al. "The receptor tyrosine kinase EphB2 regulates NMDA-dependent synaptic function" Neuron. 2001 Dec 20;32(6):1041-56 PMID:11754836 |
| Figure(s): | Figs. 1, 3, 4, Table 1 |
| Annotation description: | Absence of EphB2 results in a reduction of functional synaptically localized NMDARs Fig. 3F. Synaptic NMDA-mediated current was reduced in dentate granule neurons in EphB2 null mice. Fig. 4 and quantification in Table 1. Immunogold labeling of NMDAR1 in dendritic spines of EphB2-/- dentate granule neurons showed a 40% reduction in NMDAR1 levels associated with the postsynaptic density (PSD). Overall NMDAR1 labeling within dentate dendrites compared with controls were normal, and total hippocampal levels of NMDAR1 were similar between control and EphB2-/-. Fig 1. No expression abnormalities for NMDAR1 in EphB2 KO mice, in agreement with the overall normal NMDAR1 labeling in Fig.4 |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Confocal Microscopy (generic) Field recordings Electrophysiology (generic) |
| Annotator(s): | Tony Cijsouw (ORCID:0000-0003-0912-1514) Thomas Biederer (ORCID:0000-0002-3670-7863) |
| Lab: | Department of Neuroscience, Tufts University School of Medicine, Boston, MA 02111, USA |
| Additional literature: | EphB2 signaling through ephrins: Co-association of EphB2 Receptor and NR1 in Heterologous Cells and in Rat Cortex. NR1 co-immunoprecipitated with EphB2 only following activation of EphB2 with aggregated ephrinB1-Fc or ephrinB2-Fc. After 5 min of exposure of neurons to ephrinB1, EphB was tyrosine phosphorylated and formed clusters along neurites and cell bodies of DIV-1 neurons (Figure 3A) @ PMID:11136979 Pre-synaptic localization of ephrinB1: The authors write: "we also found that ephrinB1 and ephrinB3 are enriched in synapses at 8 and 22 DIV neurons (data not shown) (Fig. 3g). These data are consistent with a model in which postsynaptic EphB2 binds axonal ephrinB trans-synaptically to induce recruitment of presynaptic vesicles to interaction sites." @ PMID:17122040 Pre-synaptic localization of ephrinB1: Ephrin-B1 was highly colocalized with VGlut1 (45%) in confocal images of immunostained cultured cortical cultures. @ PMID:19915143 |
| SynGO annotation ID: | 279 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |