| Annotated protein: | Rho guanine nucleotide exchange factor 7 (Beta-Pix) (PAK-interacting exchange factor beta). Gene symbol: ARHGEF7. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: O55043 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ARHGEF7 |
| Ontology domain: | Biological Process |
| SynGO term: | presynaptic actin cytoskeleton organization (GO:0099140) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Sun Y, et al. "beta-Pix modulates actin-mediated recruitment of synaptic vesicles to synapses" J Neurosci. 2011 Nov 23;31(47):17123-33 PMID:22114281 |
| Figure(s): | Fig.5, 6 |
| Annotation description: | Background: IntDen=the product of an area and the average gray value within that area [Fig.2: "The density and IntDen of GFP-actin and Syn-RFP clusters are increased following ALLN treatment" - "Together, these results demonstrate that ALLN treatment enhances the accumulation of actin at discrete sites along the axon and mediates the recruitment of SVs to these sites."] Fig.5: "The IntDen of synaptic GFP-actin clusters is decreased in neurons with reduced β-pix activity." Fig.6: "The localization of synaptic vesicles at synapses is disrupted in neurons with reduced β-pix activity" |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA Over-expression |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2789 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |