| Annotated protein: | Pro-neuregulin-1, membrane-bound isoform (Pro-NRG1) [Cleaved into: Neuregulin-1 (Acetylcholine receptor-inducing activity) (ARIA) (Glial growth factor) (Heregulin) (HRG) (Neu differentiation factor) (Sensory and motor neuron-derived factor)]. Gene symbol: NRG1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q6DR98 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ NRG1 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of presynaptic active zone membrane (GO:0099059) |
| Synapse type(s): | Cerebellar mossy fiber to granule cell synapse |
| Annotated paper: | Ozaki M, et al. "Roles of neuregulin in synaptogenesis between mossy fibers and cerebellar granule cells" J Neurosci Res. 2000 Mar 1;59(5):612-23 PMID:10686589 |
| Figure(s): | Fig.7 |
| Annotation description: | Fig. 7: "An electron micrograph of the synaptic junction in cerebellar sections. Subcellular localization of NRGb1. Immuno-gold electron microscope analysis using ANB101 (A and B) and ANB102 (C) antibodies revealed that NRGb1 molecules were localized in a glomerulus of the granular layer, either postsynaptically in the dendritic profiles(A), or presynaptically in the mossy fiber terminals (B), and occasionally in the synaptic clefts (C)." Summary: the authors show expression of NRG1 in pontine nuclei (presynaptic neurons) and cerebellar granule cells (postsynaptic neurons). Immuno-EM shows NRG1 localization in pre-and postsynaptic elements located in the granular layer of the cerebellum (has mossy fiber/granule cell synapses). Sometimes gold particles are found in the synaptic cleft. Fig.3 NRG1 is shown to possess homophilic adhesive properties. The model is that some NRG1 is located at the pre- and postsynaptic specialization membrane and has a function in membrane adhesion. The authors also show shedding of extracellular domain of NRG1 by neurons. The localization to the synaptic cleft is inferred based on this. - Based on the known transmembrane topology of the protein, the "integral component of ... membrane" term was chosen. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2777 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |